Related Experiment Video
Updated: Aug 4, 2026

Real-time Live Imaging of T-cell Signaling Complex Formation
Published on: June 23, 2013
Leukocyte function-associated antigen 1 (LFA-1) is a signaling molecule for cytoskeletal changes in a human T cell
D Kelleher1, A Murphy, D Cullen
1Department of Clinical Medicine, Trinity College Dublin, St. James's Hospital, Ireland.
Abstract:
The HuT 78/2 clone is a human T cell clone which has an activated phenotype. When this line was stimulated with immobilized antibody to LFA-1, the majority of cells developed dendritic processes. These processes were demonstrated to contain cytoskeletal elements including microtubules and intermediate filaments. Phorbol myristate acetate synergized with and prolonged this effect. Thus, the LFA-1 molecule may function as a signaling molecule for cytoskeletal reorganization in a human T cell clone.
Related Concept Videos
Activation of Integrins
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
Intracellular Signaling Affects Focal Adhesions
Some...
Immunoglobulin-like Cell Adhesion Molecules
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

