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A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
Published on: January 27, 2021
Small non-coding RNA SraG regulates the operon YPK_1206-1205 in Yersinia pseudotuberculosis
1Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, The Chinese Academy of Sciences, Wuhan, China.
Abstract:
Small non-coding RNAs (sRNAs) are important post-transcriptional regulators in prokaryotes and have been demonstrated to participate in most of the cellular processes. SraG is an sRNA found in several enterobacterial species, but its targets have not been characterized. Here, we compared the protein expression patterns between the wild-type and an sraG-depleted mutant of Yersinia pseudotuberculosis by proteomic analysis. Sixteen proteins were up- or downregulated, and the negative regulatory role of SraG associated with the YPK_1206-1205 operon was confirmed. A region in the coding sequence of YPK_1206 was further demonstrated to be required for this negative regulation.
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