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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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Electrochemical amplified detection of Hg2+ based on the supersandwich DNA structure

Guangfeng Wang1, Xiuping He, Baojuan Wang

  • 1Key Laboratory of Chem-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, Anhui province, P R China. wangyuz@mail.ahnu.edu.cn

The Analyst
|March 21, 2012
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Southern Blot02:57

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...