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Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

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Multicolour single molecule imaging on cells using a supercontinuum source.

Stephen E D Webb, Laura Zanetti-Domingues, Benjamin C Coles

Biomedical Optics Express

|March 22, 2012

Summary

This summary is machine-generated.

Multicolour single molecule fluorescence imaging allows studying multiple membrane proteins in living cells. A supercontinuum laser offers flexibility and ease of use for advanced cellular membrane research.

Keywords:

(110.4234) Multispectral and hyperspectral imaging (170.2520) Fluorescence microscopy

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Area of Science:

Cellular biology
Biophysics
Optical imaging

Background:

Multicolour single molecule fluorescence imaging is crucial for studying protein interactions within living cell membranes.
Existing methods often face limitations due to constrained laser wavelength availability.

Purpose of the Study:

To introduce and evaluate a supercontinuum laser as a versatile excitation source for multicolour single molecule fluorescence imaging.
To demonstrate the applicability of this technique for analyzing membrane proteins, specifically the ErbB receptor family.

Main Methods:

Utilizing a supercontinuum laser as the excitation source for single molecule fluorescence imaging.
Comparing the performance of the supercontinuum laser with multiplexed single-wavelength lasers.
Applying the technique to observe membrane proteins in living cells.

Main Results:

The supercontinuum laser provides comparable results to traditional multiplexed lasers.
The system successfully enabled the study of membrane proteins, including the ErbB receptor family.
Demonstrated ease of use and flexibility in dye selection compared to fixed-wavelength lasers.

Conclusions:

Supercontinuum white-light sources offer significant advantages for single molecule fluorescence imaging.
This technology enhances the ability to study complex cellular processes involving membrane proteins.
Provides researchers with greater freedom in experimental design for live-cell imaging.