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Waveguide-mode sensors as aptasensors.

Subash C B Gopinath1, Koichi Awazu, Makoto Fujimaki

  • 1Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan. gopi-subashchandrabose@aist.go.jp

Sensors (Basel, Switzerland)
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Summary
This summary is machine-generated.

Aptamers, artificial nucleic acid ligands, can be used in aptasensors for diagnostics. This work reviews strategies for aptamer analysis using sensitive waveguide-mode sensors.

Keywords:
SELEXaptameraptasensoropticswaveguide

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Aptamers are artificial nucleic acid ligands selected through in vitro methods.
  • They offer potential as alternatives to antibodies and drugs in diagnostics.
  • Aptasensors are crucial for developing new diagnostic tools.

Purpose of the Study:

  • To provide an overview of strategies for aptamer-based analyses.
  • To highlight the applications of waveguide-mode sensors in aptamer detection.
  • To discuss the advantages of waveguide-mode sensors for biomolecular interaction analysis.

Main Methods:

  • In vitro selection for aptamer generation.
  • Development and application of waveguide-mode sensors.
  • Utilizing physical and chemical stability for enhanced sensitivity.

Main Results:

  • Waveguide-mode sensors are effective for analyzing biomolecular interactions, including aptamers.
  • Sensitivity can be enhanced by perforating the waveguide layer or using labels like dyes or nanoparticles.
  • The developed sensors offer physical and chemical stability.

Conclusions:

  • Waveguide-mode sensors are a viable platform for aptamer-based analyses.
  • These sensors offer a sensitive and stable method for aptasensor development.
  • Further applications in diagnostics are anticipated.