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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
08:12

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

Post-translational modifications of nuclear receptors and human disease

Muralidharan Anbalagan1, Brandy Huderson, Leigh Murphy

  • 1Department of Structural and Cellular Biology, Tulane University School of Medicine, New Orleans, Louisiana, USA.

Nuclear Receptor Signaling
|March 23, 2012
PubMed

Insights

Nuclear receptor (NR) post-translational modifications (PTMs) significantly influence physiological processes and disease development. PTMs like phosphorylation, acetylation, and sumoylation of key NRs are crucial biomarkers for disease progression and treatment response.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • Nuclear receptors (NRs) regulate vital physiological functions, including metabolism and homeostasis.
  • Post-translational modifications (PTMs) critically control NR activity and function.
  • Aberrant NR PTMs are increasingly implicated in the pathogenesis of various human diseases, notably cancer.

Purpose of the Study:

  • To review the role of PTMs in NR function.
  • To highlight the association of NR PTMs with human diseases, particularly cancer.
  • To explore the potential of NR PTMs as biomarkers for disease and treatment response.

Main Methods:

  • Literature review focusing on studies investigating NR PTMs.
  • Analysis of evidence linking specific PTMs (phosphorylation, acetylation, sumoylation) of key NRs (AR, ERα, GR, PPARγ) to disease.
  • Evaluation of PTMs as potential diagnostic and predictive markers.

Main Results:

  • Phosphorylation, acetylation, and sumoylation of androgen receptor (AR), estrogen receptor α (ERα), glucocorticoid receptor (GR), and peroxisome proliferator activated receptor γ (PPARγ) are linked to various diseases.
  • Specific PTMs of AR, ERα, GR, and PPARγ correlate with cancer progression, treatment outcomes (e.g., tamoxifen therapy), and metabolic/inflammatory conditions.
  • NR PTMs demonstrate potential as biomarkers for disease status and patient response to therapies.

Conclusions:

  • NR PTMs are critical regulators of NR function with significant implications in human health and disease.
  • The study of NR PTMs offers valuable insights into disease mechanisms and identifies potential therapeutic targets.
  • NR PTMs serve as promising biomarkers for predicting disease progression and guiding patient treatment strategies.

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