Characterization and optimization of Fe(II)Cit-No reduction by Pseudomonas sp

Area of Science:

• Environmental Science
• Microbiology
• Chemical Engineering

Background:

• Nitrogen oxide (NOx) removal from flue gases is critical for environmental protection.
• Integrated chemical absorption-biological reduction techniques utilize microbial processes for NOx abatement.
• Biological reduction of nitric oxide (NO) chelated by ferrous iron is a key step in these integrated systems.

Purpose of the Study:

• To investigate the bioreduction of nitric oxide (NO) chelated by iron(II) citrate (Fe(II)Cit-NO) using a newly isolated Pseudomonas sp. strain.
• To identify optimal conditions for the bioreduction process.
• To assess the strain's ability to utilize glucose and reduce other iron citrate complexes.

Main Methods:

• Isolation and identification of a novel Pseudomonas sp. strain.
• Bioreduction experiments of Fe(II)Cit-NO under anaerobic conditions.
• Optimization studies for factors affecting NO reduction.
• Investigation of glucose utilization and reduction of Fe(III) citrate.

Main Results:

• The isolated Pseudomonas sp. strain effectively reduced 65.9% of NO (at 670 mg/m3) within 25 hours under anaerobic conditions.
• Glucose was identified as an efficient carbon source for the bioreduction of Fe(II)Cit-NO.
• The strain demonstrated the ability to reduce Fe(III) citrate, and nitrite ions were found to inhibit cell growth and Fe(III) reduction.

Conclusions:

• The study provides valuable data on the bioreduction of Fe(II)Cit-NO by Pseudomonas sp.
• Findings support the application of this strain in NOx removal processes and scrubber solution regeneration.
• Understanding the impact of nitrite is essential for optimizing bioreactor design and operation.