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Updated: May 23, 2026

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
Published on: March 1, 2012
Non-template functions of the viral RNA in plant RNA virus replication
Kunj B Pathak1, Judit Pogany, Peter D Nagy
1Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.
Abstract:
In addition to the essential function of the viral RNA as a template during replication of positive-stranded (+)RNA viruses, the RNA also has crucial non-template functions. These functions during replication include the recruitment of the viral RNA to the site of replication and assembly of the functional viral replicase complex. The RNA recruitment elements are specifically recognized by the viral replication protein, but also affected by host factors such as elongation factor 1A or P-body proteins. The cis-elements for replicase assembly can partially overlap with RNA recruitment elements and they may provide a platform for the assembly of the replicase complex consisting of viral and host proteins. This review focuses on our current knowledge obtained with tombusviruses and other plant viruses. Altogether, understanding of the non-template functions of the viral RNA during viral replication provides new insights into virus-host interactions.
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