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Updated: May 23, 2026
Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
Published on: April 1, 2019
Optimization of a method for preparation and repopulation of the tracheal matrix for allogenic transplantation
M V Kiselevsky1, N Yu Anisimova, O V Lebedinskaya
1N. N. Blokhin Russian Oncological Research Center, Russian Academy of Medical Sciences, Moscow, Russia. kisele@inbox.ru
Abstract:
A method for decellularization of the trachea for subsequent repopulation with allogenic MSC was optimized. Tracheas from C57BL/6 mice were devitalized and repopulated with MSC from BALB/c mice. The tracheal matrix with devitalized mucosa and intact cartilaginous structure, fit for repopulation with allogenic MSC, was obtained by chemical treatment with NaClO4. This approach seemed to be promising for transplantation of allogenic trachea.
