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Updated: Feb 17, 2026

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
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Aurora kinase-C-T191D is constitutively active mutant

Jabbar Khan1, Sanaullah Khan, Sobia Attaullah

  • 1Department of Biological Sciences, Gomal University Dera Ismail Khan, Dera Ismail Khan, Pakistan.

BMC Cell Biology
|March 27, 2012
PubMed
Abstract

Insights

The Aurora kinase C-T191D mutant exhibits constitutive activity, similar to wild-type Aurora kinase C. Overexpression of this oncogenic kinase drives tumor formation and aggressiveness, suggesting it as a potential anti-cancer target.

Area of Science:

  • Cell Biology
  • Molecular Oncology
  • Cancer Therapeutics

Background:

  • Aurora kinases (A, B, C) are serine/threonine kinases regulating cell cycle progression.
  • Aurora C is typically meiotic, but overexpressed in cancers, suggesting a role beyond meiosis.
  • The Aurora-C-T191D variant was investigated for its activity and oncogenic potential.

Purpose of the Study:

  • To characterize the Aurora-C-T191D variant and compare it to wild-type Aurora kinase C.
  • To determine if Aurora-C-T191D is a hyperactive mutant.
  • To evaluate the oncogenic potential and therapeutic implications of Aurora kinase C activity.

Main Methods:

  • Overexpression of Aurora-C-T191D and wild-type Aurora kinase C in NIH-3T3 cells.
  • Assessment of cell division abnormalities (centrosome amplification, multinucleation).
  • Soft agar colony formation assays and tumor induction in nude mice.

Main Results:

  • Aurora-C-T191D overexpression mimicked wild-type Aurora kinase C behavior.
  • Both variants induced abnormal cell division and centrosome amplification.
  • Overexpression led to cellular transformation, tumor formation, and correlated aggressiveness with kinase activity.

Conclusions:

  • Aurora-C-T191D is a constitutively active mutant, not hyperactive.
  • Active Aurora kinase C possesses oncogenic properties and can transform cells.
  • Aurora kinase C is a potential therapeutic target for cancer treatment.

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