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Related Experiment Video

Updated: May 23, 2026

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Published on: August 7, 2012

Method development for proteome stabilization in human saliva.

Hua Xiao¹, David T W Wong

¹Dental Research Institute, University of California, Los Angeles, CA 90095, USA. xiaohuacn@gmail.com

Analytica Chimica Acta

|March 27, 2012

Summary

This summary is machine-generated.

Saliva protein degradation is a challenge for diagnostics. Adding ethanol stabilizes saliva proteins at room temperature for two weeks, enabling easier clinical use.

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Last Updated: May 23, 2026

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Collection and Extraction of Saliva DNA for Next Generation Sequencing

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

Area of Science:

Biochemistry
Proteomics
Clinical Diagnostics

Background:

Human saliva is a valuable source for early disease detection.
Salivary proteome degradation limits its clinical application.
Need for practical saliva sample storage methods.

Purpose of the Study:

To develop a reliable method for stabilizing human saliva proteins.
To assess protein stability under different storage conditions.
To ensure methods do not hinder downstream applications.

Main Methods:

Saliva samples stored at room temperature (RT) and 4°C.
Protein stabilization treatments evaluated.
SDS-PAGE used for protein profiling.
Immunoassay for reference protein (β-actin, IL1β) detection.

Main Results:

Saliva proteome stable for ~2 weeks at 4°C with protease inhibitors.
Ethanol addition stabilized saliva proteome at RT.
Optimized method shows no significant protein degradation.

Conclusions:

Ethanol provides a simple, robust method for saliva protein stabilization at RT.
This method enhances the utility of saliva for clinical diagnostics.
Stabilized saliva samples accelerate diagnostic applications.