Glucose biosensor based on three dimensional ordered macroporous self-doped polyaniline/Prussian blue bicomponent...

Area of Science:

• Electrochemistry
• Materials Science
• Biosensors

Background:

• Prussian blue (PB) is a redox mediator with limited stability in neutral/alkaline solutions.
• Self-doped polyaniline (SPAN) offers protection and enhanced conductivity.
• Combining SPAN and PB in a 3D ordered macroporous (3DOM) structure can overcome limitations.

Purpose of the Study:

• To fabricate a novel 3DOM SPAN/PB bicomponent film.
• To develop a highly sensitive and stable glucose biosensor using this film.
• To optimize and evaluate the performance of the glucose biosensor.

Main Methods:

• Fabrication of 3DOM SPAN/PB film via inverted crystal template and electrodeposition.
• Optimization of experimental conditions for glucose detection.
• Characterization of the biosensor's performance (linear range, detection limit, selectivity, stability).

Main Results:

• The 3DOM SPAN/PB film exhibited a large active surface area and excellent conductivity.
• The biosensor demonstrated a wide linear range (2–1600 μM) and a low detection limit (0.4 μM).
• The developed glucose biosensor showed high selectivity, short response time, and excellent operational stability.

Conclusions:

• The 3DOM SPAN/PB bicomponent film is a promising material for glucose biosensor fabrication.
• The developed glucose biosensor offers superior performance and stability.
• This biosensor can be applied for direct blood glucose monitoring in real samples without pretreatment.