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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Global relative and absolute quantitation in microbial proteomics.

Andreas Otto1, Jörg Bernhardt, Michael Hecker

  • 1Institute for Microbiology, Ernst Moritz Arndt University Greifswald, F-L-Jahn-Strasse 15, 17487 Greifswald, Germany.

Current Opinion in Microbiology
|March 27, 2012
PubMed
Summary
This summary is machine-generated.

This review covers proteomic techniques for identifying and quantifying proteins. It details current workflows for microbial physiology studies, focusing on protein abundance and changes over time.

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Area of Science:

  • Proteomics
  • Microbial Physiology
  • Biochemistry

Background:

  • Proteomic studies provide insights into protein identification, distribution, modifications, interactions, structure, and function.
  • Both qualitative and quantitative data can be obtained using various gel-based and gel-free techniques.
  • Understanding protein dynamics is crucial in microbial physiology.

Purpose of the Study:

  • To review current proteomic workflows for global protein quantitation.
  • To discuss the application of these workflows in microbial physiology.
  • To cover both relative and absolute protein quantitation methods.

Main Methods:

  • Summary of gel-based proteomic techniques.
  • Overview of gel-free proteomic techniques.
  • Discussion of workflows for relative and absolute protein quantitation.

Main Results:

  • Current proteomic workflows enable comprehensive protein analysis.
  • Various techniques allow for detailed qualitative and quantitative protein profiling.
  • These methods are applicable to understanding microbial physiology.

Conclusions:

  • Proteomic studies offer valuable qualitative and quantitative data on proteins.
  • A range of techniques are available for protein analysis.
  • Current workflows are well-suited for applications in microbial physiology.