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Exudation induces clustering of CR1 receptors at the surface of human polymorphonuclear leukocytes

J P Paccaud1, J L Carpentier, J A Schifferli

  • 1Institute of Histology and Embryology, University Medical Center, Geneva, Switzerland.

Biochemical and Biophysical Research Communications
|November 15, 1990
PubMed

Insights

Complement receptor type 1 (CR1) clustering on neutrophils increases with density in exudates but doesn't enhance immune complex binding. In vivo CR1 clustering may influence phagocytosis, independent of known activators.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Complement receptor type 1 (CR1) plays a role in immune adherence and phagocytosis.
  • Neutrophil activation influences CR1 expression and function.

Purpose of the Study:

  • To investigate CR1 surface distribution, density, and immune adherence efficiency on activated and exudate neutrophils.
  • To compare CR1 behavior on activated neutrophils with resting neutrophils.

Main Methods:

  • Assessed CR1 surface distribution, density, and immune adherence efficiency on peripheral blood neutrophils (PMN) and exudate PMN.
  • Stimulated PMN with various activators including fMLP, NAP-1/IL-8, TNF, GM-CSF, and C5a.
  • Compared CR1 behavior on resting versus activated/exudate PMN.

Main Results:

  • Neutrophil activators did not significantly increase CR1 clustering or immune adherence efficiency.
  • Exudate PMN showed increased CR1 density and clustering, but this did not enhance immune complex binding.
  • CR1 expression on exudate neutrophils could not be further increased by fMLP or PMA stimulation.

Conclusions:

  • CR1 clustering on neutrophils can occur in vivo, particularly in exudates.
  • This in vivo CR1 clustering may impact the phagocytic capacity of neutrophils for opsonized particles.
  • The observed CR1 clustering was not attributable to the specific PMN activators tested.

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