Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phenoxazines with a Phototransferable <i>N</i>-Acetyl Group and Acrylate Linker: Assembly by C-H Activation, Photoconversion to Fluorescent Dyes, Biolabeling, and Super-Resolution Imaging.

Journal of the American Chemical Society·2026
Same author

Synthesis of Benzo[<i>b</i>]thiophene 1,1-Dioxides via Pd-Catalyzed Sulfinylation of Aryl Triflates and Their Use as Large Stokes Shift Fluorophores for Multicolor Live-Cell Imaging with Self-Labeling Tags.

JACS Au·2026
Same author

Photoactivatable Carborhodol and Carborhodamine Dyes with One Cleavable Group: Synthesis, Spectra, and Fluorescence Nanoscopy Applications.

JACS Au·2026
Same author

Fluorescent Diarylethenes With Polar Groups: Synthesis, Spectra, and Optical Microscopy Applications.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same author

Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking.

Science advances·2025
Same author

The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Long-term stabilization of intensity-difference squeezing from four-wave mixing in rubidium vapor.

Optics express·2026
Same journal

Robust 3D topography measurement of large-range high-aspect-ratio structures based on dual-domain statistical filtering in SD-OCT.

Optics express·2026
Same journal

Broadband transmissive terahertz metasurface for simultaneous quad-mode OAM multiplexing.

Optics express·2026
Same journal

Leveraging two-dimensional materials for high-sensitivity optical sensors: quasi-bound states in the continuum within hybrid metasurfaces.

Optics express·2026
Same journal

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026
Same journal

Robustness of parallel subnetwork-filtered diffractive deep neural networks.

Optics express·2026
See all related articles

Related Experiment Video

Updated: May 23, 2026

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

Drift estimation for single marker switching based imaging schemes.

Claudia Geisler1, Thomas Hotz, Andreas Schönle

  • 1Department of Optical Nanoscopy, Laser-Laboratory-Göttingen, Hans-Adolf-Krebs-Weg 1, D-37077 Göttingen, Germany.

Optics Express
|March 29, 2012
PubMed
Summary
This summary is machine-generated.

Optical nanoscopes achieve 20 nm resolution, but microscope instability limits performance. This study introduces a data-driven method for drift detection and correction in single marker switching imaging, matching fiducial marker precision.

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities
07:13

Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities

Published on: October 27, 2023

Related Experiment Videos

Last Updated: May 23, 2026

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
10:04

Sample Drift Correction Following 4D Confocal Time-lapse Imaging

Published on: April 12, 2014

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities
07:13

Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities

Published on: October 27, 2023

Area of Science:

  • Super-resolution fluorescence microscopy
  • Biophysics and optical imaging techniques

Background:

  • The diffraction barrier in fluorescence imaging has been overcome, enabling optical nanoscopes to achieve resolutions as fine as 20 nm.
  • Super-resolution imaging techniques often trade speed for spatial resolution, making mechanical instabilities in microscopes a significant limiting factor.
  • Accurate drift detection and correction are crucial for maintaining high resolution in advanced microscopy.

Purpose of the Study:

  • To develop a robust, data-driven method for detecting and correcting image drift in single marker switching (SMS) imaging.
  • To provide guidelines for parameter selection and quality assessment of drift analysis in super-resolution microscopy.
  • To achieve drift determination precision comparable to established fiducial marker-tracking methods.

Main Methods:

  • A fully data-driven statistical registration method for drift detection and correction.
  • Application to single marker switching (SMS) imaging schemes.
  • Minimal assumptions regarding drift enable a model-free approach, with options for integrating specific drift models.

Main Results:

  • The proposed method effectively detects and corrects mechanical instabilities in super-resolution microscopes.
  • Performance was validated on standard SMS measurements.
  • Drift determination precision achieved is comparable to that of fiducial marker-tracking methods.

Conclusions:

  • A novel, data-driven statistical registration method significantly improves the reliability of super-resolution microscopy by addressing mechanical drift.
  • The method offers a precise and adaptable solution for drift correction in SMS imaging.
  • This approach enhances the routine applicability and accuracy of advanced optical nanoscopy.