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From lateral flow devices to a novel nano-color microfluidic assay
Saied Assadollahi1, Christiane Reininger, Roland Palkovits
1Attophotonics Biosciences GmbH, Viktor Kaplan Strasse 2, A-2700 Wiener Neustadt, Austria; E-Mails: palkovits@attophotonics.com (R.P.); Reininger@attophotonics.com (C.R.); pointl@attophotonics.com (P.P.).
Researchers developed a rapid diagnostic test using Resonance-Enhanced Absorption (REA) assays integrated into microfluidics. This innovation significantly reduces detection times for biomolecular binding, offering a faster alternative to traditional lateral flow assays.
Area of Science:
- Nanotechnology and Nanosensing
- Microfluidics and Lab-on-a-Chip Devices
- Biomolecular Detection and Diagnostics
Background:
- Traditional lateral flow assays (LFAs) face performance limitations, driving innovation in diagnostic components like labels, materials, and device design.
- Resonance-Enhanced Absorption (REA) of metal nanoparticles shows promise for bio-sensing, detecting biomolecular binding interactions effectively.
- Existing REA assays often suffer from long incubation times, hindering rapid diagnostic applications.
Purpose of the Study:
- To integrate Resonance-Enhanced Absorption (REA) assays into a microfluidic diagnostic setup.
- To overcome the limitation of long incubation times associated with previous REA assays.
- To combine this integration with automated fabrication techniques for efficient diagnostics manufacturing.
Main Methods:
- Utilized PET-co-polyester as a substrate due to its suitability for roller-coating and chemical resistance.
- Employed a CO(2) laser ablation system for precise, contactless microfluidic channel fabrication, replacing traditional micro-milling.
- Integrated REA assays within the fabricated microfluidic device for biomolecular binding detection.
Main Results:
- Successfully detected biological binding within three minutes using the novel REA-microfluidic device.
- Achieved visible, colored text readout directly within the device, indicating successful binding detection.
- Demonstrated that a two-minute in-situ silver enhancement can further amplify the resonant color signal when needed.
Conclusions:
- The integration of REA assays in a microfluidic setup significantly accelerates diagnostic detection times.
- Automated fabrication techniques, including CO(2) laser ablation, enable efficient and precise manufacturing of these advanced diagnostic devices.
- This novel approach offers a rapid, visually readable diagnostic tool with potential for widespread application in biomolecular detection.
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