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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
Published on: September 19, 2017
Amperometric hydrogen peroxide biosensor based on immobilization of hemoglobin on a glassy carbon electrode modified
Xue-Cai Tan1, Jin-Lei Zhang, Sheng-Wei Tan
1College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006, China; E-Mails: zhangjl1984123@yahoo.cn (J.-L.Z); PGtansw@126.com (S.W.T.); bczdd_198571@sina.com (D.-D.Z.); HZW8188516@126.com (Z.-W.H.); miyan9@163.com (Y.M.); hzy210@163.com (Z.-Y.H.).
Abstract:
Novel magnetic Fe(3)O(4)/chitosan (CS) microspheres were prepared using magnetic Fe(3)O(4) nanoparticles and the natural macromolecule chitosan. Then, using an easy and effective hemoglobin (Hb) immobilization method, an innovative biosensor with a Fe(3)O(4)/CS-Hb-Fe(3)O(4)/CS "sandwich" configuration was constructed. This biosensor had a fast (less than 10 s) response to H(2)O(2) and excellent linear relationships were obtained in the concentration range of 5.0 × 10(-5) to 1.8 × 10(-3) M and 1.8 × 10(-3) to 6.8 × 10(-3) M with a detection limit of 4.0 × 10(-6) M (s/n = 3) under the optimum conditions. The apparent Michaelis-Menten constant K(m) was 0.29 mM and it showed the excellent biological activity of the fixed Hb. Moreover, the biosensor had long-time stability and good reproducibility. The method was used to determine H(2)O(2) concentration in real samples.
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