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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
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Ligand binding assays in the 21st Century laboratory: platforms

Franklin P Spriggs1, Zhandong Don Zhong, Afshin Safavi

  • 1Department of Pharmacokinetics, Dynamics, and Metabolism, Pfizer, Inc., Andover, Massachusetts 01810, USA. Franklin.Spriggs@pfizer.com

The AAPS Journal
|March 29, 2012
PubMed
Summary

No abstract available in PubMed .

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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...