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High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

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Published on: January 21, 2015

Imaging the material properties of bone specimens using reflection-based infrared microspectroscopy.

Alvin S Acerbo¹, G Lawrence Carr, Stefan Judex

¹Photon Sciences Directorate, Brookhaven National Laboratory, Upton, New York 11973, United States.

Analytical Chemistry

|March 30, 2012

Summary

Reflection-based Fourier transform infrared microspectroscopy (FTIRM) offers a faster alternative to transmission-based FTIRM for analyzing bone material properties. This new method eliminates the need for thin bone sections, simplifying analysis and enabling direct correlation with mechanical testing.

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Area of Science:

Biomaterials Science
Biophysics
Materials Science

Background:

Fourier transform infrared microspectroscopy (FTIRM) is crucial for mapping bone material properties.
Traditional transmission-based FTIRM requires extensive sample preparation, limiting its application.
Developing alternative FTIRM methods is essential for broader specimen analysis.

Purpose of the Study:

To theoretically and empirically demonstrate reflection-based FTIRM as a viable alternative to transmission-based FTIRM.
To reduce specimen preparation time and expand the range of specimens that can be imaged using FTIRM.
To enable direct correlation of bone's material properties with mechanical properties.

Main Methods:

Developed and validated a reflection-based FTIRM technique using mature mouse femurs.
Compared reflection FTIRM data with traditional transmission FTIRM data from adjacent bone sections.
Utilized Kramers-Kronig analysis to derive absorption coefficients from reflectance data.

Main Results:

Reflection-based FTIRM successfully mapped mineralization, carbonate substitution, and collagen cross-linking.
Spectral assignments were indistinguishable between transmission and reflection geometries.
A slight shift in apatite crystallinity parameters was observed between the two methods.

Conclusions:

Reflection-based FTIRM eliminates the need for thin bone sections, significantly reducing preparation time.
This method facilitates direct correlation with other techniques like nanoindentation and qBSE.
Reflection-based FTIRM provides a framework for correlating bone's material and mechanical properties.