Related Experiment Video
Updated: May 23, 2026
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
Published on: December 28, 2017
Validation of the PCR-dHPLC method for rapid identification of Candida glabrata phylogenetically related species in
O Telleria1, G Ezpeleta, O Herrero
1Department of Clinical Microbiology and Infection Control, Basurto Hospital, Avenida Montevideo 18, 48013 Bilbao, Spain. otelleria001@ikasle.ehu.es
Abstract:
Since two new species phylogenetically related to Candida glabrata with slightly different phenotypes and antifungal susceptibility profiles have been described, it seems to be necessary from clinical point of view, to develop a rapid and accurate identification system in order to distinguish between these three fungal species. We studied the performance of denaturing high performance liquid chromatography (dHPLC) as a faster (less than 7 min) and alternative novel technique for simultaneous analysis of Candida species in different biological matrices. The analyses show the good low limit of detection (LLOD) in all biological matrices studied (5.16-9.56 ngμL(-1), 4.14-4.70 ng μL(-1) and 3.99-4.66 ng μL(-1) for Candida bracarensis, Candida nivariensis and C. glabrata, respectively). 180 Candida isolates were analyzed in order to demonstrate the method suitability for screening analysis to identify C. glabrata and its cryptic species (C. bracarensis and C. nivariensis) in clinical routine.
