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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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Cryo-EM--the first thirty years

J Dubochet1

  • 1DEE, Biophore, Faculty of Biology andMedicine, University of Lausanne, CH-1015 Lausanne, Switzerland. Jacques.Dubochet@unil.ch

Journal of Microscopy
|March 30, 2012
PubMed

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Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...