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Updated: May 23, 2026
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
A novel asymmetric-loop molecular beacon-based two-phase hybridization assay for accurate and high-throughput
Qinghai Chen1, Nan Wu, Meng Xie
1Clinical Experimental Laboratory of Biosensor and Microarray, and Center of Molecular and Genetic Diagnosis, Southwest Hospital, 3rd Military Medical University, Chongqing, PR China.
Abstract:
The accurate and high-throughput detection of drug resistance-related multiple point mutations remains a challenge. Although the combination of molecular beacons with bio-immobilization technology, such as microarray, is promising, its application is difficult due to the ineffective immobilization of molecular beacons on the chip surface. Here, we propose a novel asymmetric-loop molecular beacon in which the loop consists of 2 parts. One is complementary to a target, while the other is complementary to an oligonucleotide probe immobilized on the chip surface. With this novel probe, a two-phase hybridization assay can be used for simultaneously detecting multiple point mutations. This assay will have advantages, such as easy probe availability, multiplex detection, low background, and high-efficiency hybridization, and may provide a new avenue for the immobilization of molecular beacons and high-throughput detection of point mutations.
