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Related Experiment Video

Updated: Mar 28, 2026

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

Fast optically sectioned fluorescence HiLo endomicroscopy.

Tim N Ford¹, Daryl Lim, Jerome Mertz

¹Boston University, Department of Biomedical Engineering, Boston, Massachusetts 02215, USA. timford@bu.edu

Journal of Biomedical Optics

|April 3, 2012

Summary

This summary is machine-generated.

We developed a new fiber bundle endomicroscope using HiLo imaging for fast, optically sectioned fluorescence imaging. This technique offers high resolution and reduced motion artifacts, making it suitable for real-time clinical optical biopsies.

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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

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Published on: January 12, 2011

Area of Science:

Biomedical optics
Medical imaging technology
Microscopy

Background:

Flexible fiber bundle endomicroscopy enables in vivo imaging.
Optical sectioning is crucial for high-resolution microscopy.
Existing methods may suffer from motion artifacts and slow processing.

Purpose of the Study:

To present an improved nonscanning fiber bundle endomicroscope.
To achieve fast, optically sectioned fluorescence imaging with real-time processing.
To demonstrate the utility of HiLo imaging for endomicroscopy.

Main Methods:

Utilized HiLo imaging combining uniform and structured illumination.
Employed a graphics processing unit (GPU) for real-time image processing.
Incorporated a double-shutter camera to minimize motion artifacts.

Main Results:

Achieved lateral and axial resolutions of 2.6 μm and 17 μm, respectively.
Attained a net frame rate of 9.5 Hz through GPU processing.
Demonstrated successful imaging of fluorescently labeled chorioallantoic membrane (CAM) in G. gallus embryos.

Conclusions:

The improved HiLo endomicroscope provides fast, high-resolution optical sectioning.
The system effectively reduces motion artifacts for clearer imaging.
This technique is a promising candidate for low-cost, high-speed clinical optical biopsies.