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High-performance liquid chromatography for the separation of angiotensin and its metabolites in human plasma and
K Hermann1, J Ring, M I Phillips
1Department of Dermatology, Ludwig Maximilians University Münich, West Germany.
Journal of Chromatographic Science
|October 1, 1990
Abstract:
A reversed-phase high-performance liquid chromatography (HPLC) method with gradient elution for the separation of angiotensin peptides is described. The highly reproducible method allows the base-line separation of angiotensin peptides with UV detection at 225 nm. This chromatographic methodology in combination with radioimmunoassay (RIA) is used for the characterization of angiotensin peptides in human plasma and sweat.