Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 23, 2026

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
12:08

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

Published on: March 10, 2016

Culturing embryonic nasal explants for developmental and physiological study.

Ulrike Klenke1, Carol Taylor-Burds

  • 1Cellular and Developmental Neurobiology Section, NIH/NINDS, Bethesda, Maryland, USA.

Current Protocols in Neuroscience
|April 4, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond Alzheimer's disease-translating biomarker insights across CNS diseases.

Science translational medicine·2025
Same author

Becoming a resilient scientist series: An intervention program.

medRxiv : the preprint server for health sciences·2023
Same author

Pre-Clinical Common Data Elements for Traumatic Brain Injury Research: Progress and Use Cases.

Journal of neurotrauma·2020
Same author

FAIR SCI Ahead: The Evolution of the Open Data Commons for Pre-Clinical Spinal Cord Injury Research.

Journal of neurotrauma·2019
Same author

CCDC141 Mutation Identified in Anosmic Hypogonadotropic Hypogonadism (Kallmann Syndrome) Alters GnRH Neuronal Migration.

Endocrinology·2016
Same author

BPA Directly Decreases GnRH Neuronal Activity via Noncanonical Pathway.

Endocrinology·2016

This study presents a novel nasal explant culture system for studying olfactory neurons and gonadotropin-releasing hormone-1 (GnRH) neurons. The method allows detailed examination of cell interactions, migration, and axonal outgrowth in a simplified tissue model.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Embryonic nasal placodes contain olfactory neurons, olfactory ensheathing cells, and gonadotropin releasing hormone-1 (GnRH) neurons.
  • Studying cell interactions during placode formation, neuronal migration, and axonal outgrowth is crucial for understanding neurodevelopment.

Purpose of the Study:

  • To describe a novel system for culturing embryonic nasal explants.
  • To provide a model for investigating cell interactions, neuronal migration, and axonal outgrowth.
  • To enable physiological and molecular studies on nasal placode-derived cells.

Main Methods:

  • Primary cultures of embryonic mouse nasal placodes were established.
  • Explants were generated at embryonic day 11.5 (E11.5) to facilitate imaging and manipulation.

More Related Videos

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme
07:45

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme

Published on: January 20, 2013

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
10:38

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

Published on: October 8, 2013

Related Experiment Videos

Last Updated: May 23, 2026

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
12:08

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells

Published on: March 10, 2016

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme
07:45

An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme

Published on: January 20, 2013

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
10:38

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

Published on: October 8, 2013

  • The system allows for pharmacological, molecular, and physiological investigations.

    • Main Results:

    • The nasal explant culture maintains olfactory neurons, olfactory ensheathing cells, and GnRH neurons.
    • Neuronal migration and axonal outgrowth occur at the periphery of E11.5 explants, creating a thin layer for study.
    • The model system enables physiological cellular responses to be recorded.

    Conclusions:

    • The described nasal explant culture system is a valuable tool for studying nasal placode development.
    • This model facilitates detailed analysis of cell interactions, neuronal migration, and axonal outgrowth.
    • The system supports post hoc analyses such as immunohistochemistry and gene expression studies.