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The PRE and PQ box are functionally distinct yeast pheromone response elements.

P Sengupta1, B H Cochran

  • 1MIT Center for Cancer Research, Cambridge.

Molecular and Cellular Biology
|December 1, 1990
PubMed
Summary
This summary is machine-generated.

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The study identified distinct DNA sequences, the PRE and PQ box, that control yeast mating pheromone responses. These elements show opposite cell-type specificities, highlighting unique regulatory mechanisms in Saccharomyces cerevisiae.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Cell Signaling

Background:

  • Saccharomyces cerevisiae mating pheromones regulate gene expression via cell surface receptors and signal transduction.
  • Understanding the specific DNA sequences that confer pheromone inducibility is crucial for deciphering gene regulation.

Purpose of the Study:

  • To determine the minimal sequence requirements for conferring both a and alpha mating pheromone inducibility onto a heterologous promoter.
  • To investigate the distinct roles and cell-type specificities of pheromone-responsive elements.

Main Methods:

  • Analysis of heterologous promoters containing specific DNA sequences (PRE and PQ box).
  • Assessing pheromone inducibility in different yeast cell types (a and alpha cells).
  • Investigating protein binding (STE12, MCM1, MAT alpha 1) to these elements.

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Main Results:

  • The pheromone response element (PRE) requires multiple copies for inducibility and is preferentially responsive to alpha factor in a cells.
  • A single copy of the PQ box confers a-factor responsiveness in alpha cells but not alpha-factor responsiveness in a cells.
  • The PQ box binds MCM1 and MAT alpha 1 cooperatively, with neither site alone being sufficient.

Conclusions:

  • The PRE and PQ box are functionally distinct pheromone-responsive elements with opposite cell-type specificities.
  • MCM1 protein acts in a signal transduction pathway analogous to mammalian serum response factor.
  • These findings elucidate specific DNA elements controlling yeast mating pheromone response pathways.