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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 23, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Microfluidic integration for automated targeted proteomic assays.

Alex J Hughes1, Robert K C Lin, Donna M Peehl

  • 1Department of Bioengineering, and University of California, Berkeley-University of California San Francisco Graduate Program in Bioengineering, University of California, Berkeley, CA 94720, USA.

Proceedings of the National Academy of Sciences of the United States of America
|April 5, 2012
PubMed
Summary
This summary is machine-generated.

We developed a microfluidic immunoblotting assay for scalable protein isoform analysis. This innovation enables faster development of protein diagnostics for personalized medicine and improved biomarker verification.

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Last Updated: May 23, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Personalized medicine requires precise protein isoform diagnostics, which are currently limited.
  • Efficient protein biomarker validation is crucial for developing effective diagnostics.

Purpose of the Study:

  • To introduce a scalable microfluidic strategy for protein isoform analysis.
  • To enable high-throughput targeted proteomics for personalized medicine.

Main Methods:

  • A "single-channel, multistage" microfluidic immunoblotting assay was developed.
  • The assay integrates separation, immobilization, antibody probing, and washing steps.
  • Programmable electrophoretic transport and a 3D hydrogel eliminate pumps, valves, and manual blotting.

Main Results:

  • The assay demonstrates superior performance with enhanced capture efficiency and reduced reagent consumption.
  • Achieved a low limit of detection (1.1 pg) for prostate-specific antigen isoforms in cell lysate.
  • Successfully measured endogenous prostate-specific antigen isoforms in crude patient sera.

Conclusions:

  • The integrated microfluidic assay provides a scalable framework for high-throughput proteomics.
  • This technology facilitates robust protein biomarker verification for personalized medicine.
  • Enables systematic characterization of antibody probes and biospecimen repositories.