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A relation between TGF-β and mast cell tryptase in experimental emphysema models
Esmaeil Mortaz1, Masoumeh E Givi, Carla A Da Silva
1Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, The Netherlands. emortaz@gmail.com
Biochimica Et Biophysica Acta
|April 7, 2012
Summary
Cigarette smoke exposure increases mast cells and their tryptase in emphysema models. Transforming growth factor-beta (TGF-β) mediates this effect, highlighting mast cells
Area of Science:
- Pulmonary Medicine and Pathology
- Cell Biology and Immunology
Background:
- Chronic obstructive pulmonary disease (COPD) involves emphysema and chronic bronchitis.
- Mast cells play a role in emphysema pathogenesis.
- Cigarette smoke is a primary risk factor for COPD.
Purpose of the Study:
- To investigate the effect of cigarette smoke on mast cell function.
- To elucidate the role of mast cells and TGF-β in emphysema pathogenesis.
Main Methods:
- In vitro study using primary cultured mast cells exposed to cigarette smoke conditioned medium (CSM).
- In vivo study using a mouse model of emphysema induced by cigarette smoke exposure.
- Measurement of mast cell tryptase (MMCP-6) expression and TGF-β levels.
Main Results:
- CSM induced mast cell tryptase expression in vitro.
- CSM-stimulated TGF-β production mediated tryptase expression.
- Cigarette smoke exposure increased airway mast cells and tryptase expression in vivo.
- Elevated TGF-β levels were observed in bronchoalveolar lavage fluid of smoke-exposed mice.
Conclusions:
- Mast cells are implicated in the pathogenesis of emphysema.
- Mast cells may produce TGF-β in response to cigarette smoke.
- TGF-β influences mast cell tryptase expression, contributing to emphysema development.
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