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Related Concept Videos

Allergic Reactions02:06

Allergic Reactions

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Allergic Reactions: Anaphylaxis01:30

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Anaphylaxis is a severe, life-threatening hypersensitivity reaction mediated by Immunoglobulin E (IgE) antibodies. When IgE binds to allergens, it triggers the release of mediators– histamine, leukotrienes, and prostaglandins from mast cells and basophils. These mediators cause vasodilation, edema, and inflammation, leading to various symptoms.The primary allergens causing anaphylaxis include food items (e.g., peanuts, shellfish), drugs (e.g., penicillin, asparaginase, corticotropin, heparin),...
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Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing numerous...
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Related Experiment Video

Updated: May 23, 2026

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
06:34

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

Published on: June 4, 2017

Component-resolved allergy diagnosis: a new era?

D G Ebo1

  • 1Departement Immunologie, Allergologie en Reumatologie, Universitair Ziekenhuis Antwerpen, Laboratorium voor Immunologie, Universiteit Antwerpen. Campus Drie Eiken--Universiteitsplein 1, B 2610 Antwerpen.

Verhandelingen - Koninklijke Academie Voor Geneeskunde Van Belgie
|April 10, 2012
PubMed
Summary

Component-resolved allergy diagnosis (CRD) offers a new approach to allergy testing by analyzing specific IgE antibodies to individual allergen components. While promising for patient management, further validation is needed before CRD replaces traditional methods.

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Last Updated: May 23, 2026

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
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Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Published on: April 21, 2019

Area of Science:

  • Immunology
  • Allergy Diagnostics
  • In Vitro Assays

Background:

  • Traditional allergy diagnosis relies on whole allergen extracts.
  • Component-resolved allergy diagnosis (CRD) utilizes purified or recombinant allergen components.
  • CRD quantifies specific IgE (sIgE) antibodies to individual proteins.

Purpose of the Study:

  • To introduce and evaluate component-resolved allergy diagnosis (CRD) as a novel in vitro technique.
  • To compare CRD with traditional specific IgE (sIgE) assays.
  • To assess the potential of CRD in improving allergic patient management.

Main Methods:

  • Quantification of sIgE antibodies to single protein components.
  • Utilizing purified native or recombinant allergen components.
  • Comparison with traditional sIgE assays based on whole extracts.

Main Results:

  • CRD allows for discrimination between clinically relevant and irrelevant sIgE results.
  • CRD can establish sensitization patterns with prognostic value.
  • CRD shows potential to improve allergic patient management.

Conclusions:

  • Component-resolved allergy diagnosis (CRD) is an emerging in vitro technique for allergy diagnosis.
  • CRD may enhance patient management by identifying significant allergen components.
  • Further clinical validation is required before CRD can be widely adopted or replace traditional sIgE assays.