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A selective metabolite array for the detection of phosphometabolites
Malinda Salim1, Gregory J S Fowler, Phillip C Wright
1ChELSI Institute, Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, UK.
Analytica Chimica Acta
|April 10, 2012
Summary
This study introduces a new method using immobilised metal ion affinity (IMA) to detect phosphometabolites in metabolomics. Gallium and zirconium showed high affinity, enabling the development of novel metabolite arrays for specific detection.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Materials Science
Background:
- Immobilised metal ion affinity (IMA) is traditionally used for separating phosphorylated proteins and nucleic acids.
- Its application in metabolomics for phosphometabolite detection is novel.
Purpose of the Study:
- To explore the novel application of IMA in metabolomics.
- To develop metabolite arrays for detecting phosphometabolites using plasma polymer-modified surfaces.
Main Methods:
- Immobilisation of various metal ions (gallium, zirconium, cobalt, copper, zinc, nickel, iron, chromium) onto acrylic acid plasma polymer.
- Exposure of immobilised metals to phosphometabolites and non-phosphometabolites.
- Analysis using Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS).
Main Results:
- Gallium and zirconium demonstrated superior phosphometabolite affinity and specificity compared to other tested metals.
- These metals can be effectively immobilised on plasma polymer surfaces.
Conclusions:
- Gallium and zirconium are promising candidates for developing selective metabolite arrays.
- IMA offers a new approach for phosphometabolite detection in metabolomics.
