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Urinary Peptide Signatures Distinguish Overactive Bladder and are Associated with Improvement in Incontinence
Aaron W Miller1, Stacy H Jeong2, Sean McSweeney2
1Department of Urology, Glickman Urological Institute, Cleveland Clinic, Cleveland, OH, USA; Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA.
Urology
|August 6, 2026
Summary
Overactive bladder (OAB) is linked to distinct urinary peptide patterns. These urinary peptides may predict treatment success, aiding personalized OAB therapies.
Area of Science:
- Biochemistry
- Urology
- Biomarker Discovery
Background:
- Overactive bladder (OAB) is a common condition with complex pathophysiology.
- Urinary peptide profiles are not well-characterized in OAB patients.
- Identifying biomarkers for OAB and treatment response is crucial.
Purpose of the Study:
- To characterize urinary peptide signatures in patients with and without OAB.
- To identify peptide differences based on OAB phenotype.
- To determine if baseline peptide profiles predict treatment response.
Main Methods:
- Peptidomic analysis using LC-MS/MS on urine samples from 152 participants (100 OAB patients, 52 controls).
- Statistical analyses and random forest machine learning to differentiate OAB from controls and responders from non-responders.
- Validated questionnaires assessed symptoms at baseline and post-treatment.
Main Results:
- Significant differences in urinary peptide composition between OAB patients and controls (p=0.001).
- Fibrillin-1 and quiescin sulfhydryl oxidase 1 peptides were more abundant in OAB.
- Baseline peptide profiles differed between treatment responders and non-responders (p=0.03).
Conclusions:
- OAB is associated with a unique urinary peptide signature reflecting extracellular matrix remodeling and metabolic alterations.
- Urinary peptides show potential as prognostic biomarkers for OAB treatment outcomes.
- Findings support OAB heterogeneity and may guide personalized diagnostic and therapeutic strategies.
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