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Detection of p53 gene point mutation using sequence-specific molecularly imprinted PoPD electrode
Ashutosh Tiwari1, Swapneel R Deshpande2, Hisatoshi Kobayashi3
1Biosensors and Bioelectronics Centre, Institute of Physics, Chemistry and Biology, IFM-Linköping University, S-58183 Linköping, Sweden; Linköping Integrative Regenerative Medicine (IGEN) Centre, IKE-Linköping University, S-581 83 Linköping, Sweden; International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-2-1, Sengen, Tsukuba, Ibaraki 305 0047, Japan.
A novel biosensor was developed for detecting specific DNA sequences using molecular imprinting. This sequence-specific oligodeoxyribonucleotide (ODN) biosensor offers ultra-sensitivity and selectivity for genetic analysis.
Area of Science:
- Biosensor Technology
- Molecular Imprinting
- Oligonucleotide Detection
Background:
- Developing sequence-specific biosensors is crucial for genetic analysis.
- Molecular imprinting offers a robust method for creating selective recognition sites.
Purpose of the Study:
- To fabricate and characterize an amperometric sequence-specific molecularly imprinted single-stranded oligodeoxyribonucleotide (ss-ODN) biosensor.
- To evaluate the performance of the developed biosensor for ss-ODN detection.
Main Methods:
- Fabrication of the biosensor via electropolymerization of o-phenylenediamine on an indium-tin oxide (ITO) substrate using ss-ODN as a template.
- Characterization using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and cyclic voltammetry (CV).
- Amperometric detection of target ss-ODN concentration.
Main Results:
- The biosensor demonstrated a linear amperometric response to target ss-ODN concentration from 0.01-300 fM.
- Achieved a sensitivity of 0.62 μA/fM with a rapid response time of 14 seconds.
- Exhibited high selectivity and stability.
Conclusions:
- The developed molecularly imprinted ss-ODN biosensor is highly sensitive and selective.
- This novel biosensor presents cost-effectiveness and improved storage stability.
- It holds potential for commercial genetic sensor applications.
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