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Updated: May 23, 2026

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Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill
Published on: January 29, 2021
Freeze-thaw lysis for extraction of proteins from Mammalian cells
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
CSH Protocols
|April 10, 2012
Abstract:
INTRODUCTIONThis protocol describes the extraction of proteins from mammalian cells grown in 6- or 10-cm tissue-culture dishes. It is useful for making very concentrated extracts for Western blotting, and is particularly helpful when low-abundance proteins such as Myc are being studied.

