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Updated: May 23, 2026
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Detection of Protein Ubiquitination
Published on: August 19, 2009
Detection of ubiquitylated proteins in yeast
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
CSH Protocols
|April 10, 2012
Abstract:
INTRODUCTIONThis assay is performed to detect ubiquitylated proteins in yeast. Yeast that have been transformed with a vector expressing polyhistidine-tagged ubiquitin (Ub) under the control of a copper-inducible promoter are grown, induced with copper, and harvested. Total ubiquitylated proteins are then recovered by nickel-affinity chromatography, and specific proteins are detected by Western blotting.
