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Related Experiment Video

Updated: May 23, 2026

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

Gene delivery by direct injection (microinjection) using a controlled-flow system.

David A Dean

    CSH Protocols
    |April 10, 2012
    PubMed
    Summary
    This summary is machine-generated.

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    This protocol details a simple, low-cost constant-flow microinjection method using the Pneumatic PicoPump. Sample injection volume is controlled by the duration the pipette stays within the cell.

    Area of Science:

    • Cell biology
    • Biotechnology
    • Microfluidics

    Background:

    • Microinjection is a key technique for delivering substances into cells.
    • Existing methods can be complex or expensive.
    • A need exists for simpler, cost-effective microinjection systems.

    Purpose of the Study:

    • To describe a simple and low-cost method for constant-flow microinjection.
    • To detail the use of the Pneumatic PicoPump for precise sample delivery.
    • To provide a protocol for researchers with budget constraints.

    Main Methods:

    • Utilizes a Pneumatic PicoPump system for controlled sample delivery.
    • Employs a pressure regulator for adjustable back and injection pressures.
    • Integrates a capillary holder and micromanipulator for precise positioning.

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    Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
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    Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

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    Related Experiment Videos

    Last Updated: May 23, 2026

    Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
    12:52

    Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

    Published on: May 12, 2018

    High Throughput Microinjections of Sea Urchin Zygotes
    12:40

    High Throughput Microinjections of Sea Urchin Zygotes

    Published on: January 21, 2014

    Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
    09:45

    Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata

    Published on: October 1, 2016

  • Determines injected sample volume by controlling pipette-in-cell duration.

    • Main Results:

    • The described system allows for constant sample flow from the pipette tip.
    • Injection volume is directly proportional to the time the pipette remains in the cell.
    • The setup is simple to assemble and operate.
    • The method is suitable for researchers on a limited budget.

    Conclusions:

    • This constant-flow microinjection protocol offers a simple, affordable alternative.
    • The Pneumatic PicoPump facilitates precise control over sample delivery.
    • The technique is valuable for cell biology and biotechnology applications requiring microinjection.