Related Experiment Video

Updated: May 23, 2026

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
09:31

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

Kanamycin selection of transformed Arabidopsis

Detlef Weigel, Jane Glazebrook

    CSH Protocols
    |April 10, 2012
    PubMed

    Abstract:

    INTRODUCTIONThe most commonly used markers for selection of transgenic Arabidopsis are resistance to the antibiotic kanamycin and to the herbicide glufosinate ammonium. Resistance to kanamycin is conferred by a bacterial gene encoding the enzyme neomycin phosphotransferase (NPT). In this protocol, kanamycin-resistant seedlings are selected on solid medium.

    More Related Videos

    Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
    08:13

    Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

    Published on: October 17, 2017

    Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
    05:06

    Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

    Published on: January 5, 2024

    Related Experiment Videos

    Last Updated: May 23, 2026

    Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
    09:31

    Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

    Published on: April 19, 2019

    Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
    08:13

    Standardized Method for High-throughput Sterilization of Arabidopsis Seeds

    Published on: October 17, 2017

    Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
    05:06

    Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

    Published on: January 5, 2024

    Related Concept Videos

    Antibiotic Selection00:57

    Antibiotic Selection

    Overview
    Transgenic Plants02:50

    Transgenic Plants

    Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
    The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

    Articles linked to this work by shared authors, journal, and citation graph.

    A cost-effective DNA extraction protocol for long-read sequencing in non-model plants.

    Applications in plant sciences·2026

    Biosynthetic gene clusters in Pseudomonas viridiflava have a fitness cost during Arabidopsis thaliana infection.

    mSystems·2026

    Seasonality, land use and plant community diversity shape microbiome-pathogen interactions in wild populations of Arabidopsis thaliana.

    Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026

    PLANeT: Understanding and leveraging the genome of land plants for a sustainable future.

    Cell·2026

    Rapid adaptation and extinction in synchronized outdoor evolution experiments of Arabidopsis.

    Science (New York, N.Y.)·2026

    Multiple redundant mechanisms account for the majority of gene silencing downstream of DNA methylation.

    bioRxiv : the preprint server for biology·2026

    Identification of positive GATEWAY expression clones when both the pENTRY and pDEST vectors contain the same marker for bacterial selection.

    CSH protocols·2012

    Imaging protein interactions by FRET microscopy: cell preparation for FRET analysis.

    CSH protocols·2012

    Imaging protein interactions by FRET microscopy: labeling proteins with fluorescent dyes.

    CSH protocols·2012

    Bradford assay.

    CSH protocols·2012

    Detection of ubiquitylated proteins in mammalian cells.

    CSH protocols·2012

    Imaging of organelle membrane systems and membrane traffic in living cells.

    CSH protocols·2012

    Seeds from ALS patients homozygous for the SOD1 D90A mutation transmit two types of SOD1 aggregation and motor neuron disease.

    Acta neuropathologica·2026

    Silicon Quantum Dot Seed Priming Combined with Arbuscular Mycorrhizal Fungi Enhances Wheat Drought Tolerance through Physiological and Microbiome Regulation.

    Journal of agricultural and food chemistry·2026

    Characterization and Functional Analysis of UDP-Glycosyltransferases Reveal Their Contribution to Insecticides Detoxification in Sogatella furcifera (Horváth).

    Journal of agricultural and food chemistry·2026

    Development of SNP-based markers associated with restorer-of-fertility in chilli (Capsicum annuum L.).

    Physiology and molecular biology of plants : an international journal of functional plant biology·2026

    The type II metacaspase 4 modulates wheat responses to barley stripe mosaic virus infection.

    International journal of biological macromolecules·2026

    VmUGT88A65 and VmUGT88A66 orchestrate root development and pathogen resistance in tung tree via flavonoid and lignin glycosylation.

    Plant physiology·2026
    See all related articles
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies
    Jove
    Visualize
    Contact Us