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Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
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Long-patch Base Excision Repair01:02

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Updated: May 23, 2026

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
14:06

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

Hidden treasures in unspliced EST data.

J Engelhardt1, P F Stadler

  • 1Bioinformatics Group, Department of Computer Science, and Interdisciplinary Center of Bioinformatics, University of Leipzig, Härtelstrasse 16-18, 04107 Leipzig, Germany.

Theory in Biosciences = Theorie in Den Biowissenschaften
|April 10, 2012
PubMed
Summary
This summary is machine-generated.

This study analyzes unspliced ESTs in the human genome, revealing they often cluster near genes and can form 3' UTR extensions. These findings shed light on the function of less-studied non-coding RNAs.

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

  • Genomics
  • Transcriptomics
  • Molecular Biology

Background:

  • Several classes of unspliced non-coding RNAs, including intronic and long intergenic RNAs, are known to regulate gene expression via chromatin association.
  • Intron-less protein-coding genes and unspliced non-coding transcripts remain understudied compared to spliced mRNAs.

Purpose of the Study:

  • To systematically analyze the distribution of unspliced expressed sequence tags (ESTs) in the human genome.
  • To investigate the genomic location and potential functions of these underrepresented transcripts.

Main Methods:

  • Analysis of unspliced EST distribution across the human genome.
  • Examination of EST cluster overlaps with annotated RefSeq genes.
  • Investigation of CAGE tag patterns to infer transcript structures and associations.

Main Results:

  • Most unspliced ESTs are found in clusters near or overlapping annotated RefSeq genes.
  • Partially intronic unspliced ESTs exhibit complex overlap patterns with gene intron/exon structures.
  • A significant class of unspliced EST clusters forms extended 3'UTRs, with hundreds potentially existing as independent 3'UTR-associated RNAs.

Conclusions:

  • Unspliced ESTs represent a substantial and often overlooked component of the human transcriptome.
  • These transcripts play a role in gene regulation, particularly through 3' UTR modifications and potentially as independent regulatory RNAs.
  • Further research into unspliced non-coding RNAs is warranted to fully understand their functional significance.