Related Experiment Video
Updated: May 23, 2026

07:11
Homemade Site Directed Mutagenesis of Whole Plasmids
Published on: May 11, 2009
Rapid and Efficient Site-directed Mutagenesis by the Single-tube Megaprimer PCR Method
CSH Protocols
|April 10, 2012
Abstract
No abstract available in PubMed .
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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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