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Construction of cDNA libraries * stage 3: methylation of cDNA

Joseph Sambrook, David W Russell

    CSH Protocols
    |April 10, 2012
    PubMed
    Abstract

    No abstract available in PubMed .

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    Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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