Related Experiment Video

Updated: May 23, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
10:34

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

Detection of RNAi-Induced Protein Knockdown in Mammalian Cells by Western Blotting

Thomas Tuschl

    CSH Protocols
    |April 10, 2012
    PubMed
    Abstract

    No abstract available in PubMed .

    More Related Videos

    Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
    09:39

    Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

    Published on: August 21, 2014

    RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
    14:41

    RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

    Published on: July 11, 2020

    Related Experiment Videos

    Last Updated: May 23, 2026

    Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
    10:34

    Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

    Published on: July 22, 2016

    Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
    09:39

    Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

    Published on: August 21, 2014

    RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
    14:41

    RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

    Published on: July 11, 2020

    Related Concept Videos

    Experimental RNAi02:15

    Experimental RNAi

    RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
    RNA Interference01:23

    RNA Interference

    RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
    This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

    Articles linked to this work by shared authors, journal, and citation graph.

    Rethinking the Benign Nature of Class II Lupus Nephritis.

    bioRxiv : the preprint server for biology·2026

    Coordinated regulation of mRNA translation and stability by ZC3H7A and ZC3H7B RNA-binding proteins.

    Cell reports·2026

    Dissection of innate-immune-ligand- and interferon-protein-mediated transcriptional responses in human THP1 cell states.

    Communications biology·2026

    Discovery of Novel Isofunctional SARS-CoV‑2 NSP14 RNA Cap Methyltransferase Inhibitors by Structure-Based Virtual Screening.

    ACS medicinal chemistry letters·2025

    Discovery, Optimization, and Evaluation of Non-Nucleoside SARS-CoV-2 NSP14 Inhibitors.

    Journal of medicinal chemistry·2025

    Structural insight into the cGAS active site explains differences between therapeutically relevant species.

    Communications chemistry·2025

    Identification of positive GATEWAY expression clones when both the pENTRY and pDEST vectors contain the same marker for bacterial selection.

    CSH protocols·2012

    Imaging protein interactions by FRET microscopy: cell preparation for FRET analysis.

    CSH protocols·2012

    Imaging protein interactions by FRET microscopy: labeling proteins with fluorescent dyes.

    CSH protocols·2012

    Bradford assay.

    CSH protocols·2012

    Detection of ubiquitylated proteins in mammalian cells.

    CSH protocols·2012

    Imaging of organelle membrane systems and membrane traffic in living cells.

    CSH protocols·2012

    Emerging Patterns of Mental Health Comorbidities Among Those With Blast or Blunt Mechanism TBI: Leveraging Total Brain Diagnostics Data.

    The Journal of head trauma rehabilitation·2026

    Saving the Limb-Tissue Collectors, Growth Factors, and Antibiotics in a War Injury.

    Zeitschrift fur Orthopadie und Unfallchirurgie·2026

    Integrative ALS therapy: gene targeted therapy by base editing and potential exercise rehabilitation as synergistic strategies for functional recovery.

    Physical activity and nutrition·2026

    Aging concomitantly reduces skeletal muscle proteome plasticity and hypertrophic responses to resistance training in male adults.

    Physiological reports·2026

    High Infection Rate in Open Anterior Talofibular Ligament Repair with Internal Brace Augmentation and Immediate Permissive Weight Bearing.

    The Journal of foot and ankle surgery : official publication of the American College of Foot and Ankle Surgeons·2026

    The Role of TXNIP in Skeletal Muscle Physiology and Related Diseases: Current Perspectives.

    Journal of inflammation research·2026
    See all related articles
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies
    Jove
    Visualize
    Contact Us