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Delivery of DNA to cells in culture using particle bombardment.

Methods in molecular biology (Clifton, N.J.)·2004

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Updated: May 23, 2026

Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research

Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research

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Gene delivery to skin using biolistics.

William C Heiser¹

¹Bio-Rad Laboratories, Life Science Group, Hercules, California 94547, USA.

|April 10, 2012

Summary

Biolistics, or particle bombardment, offers a fast physical method for gene delivery into cells using DNA-coated particles. This technique enables in vitro, ex vivo, and in vivo gene transfer across various cell types.

Area of Science:

Biotechnology
Molecular Biology
Genetics Engineering

Background:

Particle bombardment, also known as biolistics, is a physical method for gene delivery.
This technique offers advantages such as the use of plasmids and applicability to diverse cell types.
Gene delivery can be achieved in vitro, ex vivo, or in vivo.

Purpose of the Study:

To introduce the biolistics technique for gene delivery.
To highlight the advantages and applications of particle bombardment.
To describe the methodology of DNA-coated particle preparation and delivery.

Main Methods:

DNA-coated gold particles are prepared and distributed along tubing.
Tubing is sectioned into cartridges for use in a gene gun.

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The Helios Gene Gun utilizes a helium pulse to launch particles onto target cells.

Main Results:

Successful gene delivery to cells via particle bombardment.
Demonstration of a rapid and simple physical procedure for gene transfer.
Versatility in delivering genes to various cell types and conditions.

Conclusions:

Biolistics is an effective physical method for gene delivery.
The technique supports in vitro, ex vivo, and in vivo applications.
Particle bombardment provides a versatile tool for genetic engineering.