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Freezing Human ES Cells
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Freezing Human ES Cells

Published on: October 12, 2006

Freezing Embryonic Stem (ES) Cells in 96-Well Plates

Andras Nagy, Marina Gertsenstein, Kristina Vintersten

    CSH Protocols
    |April 10, 2012
    PubMed
    Summary

    No abstract available in PubMed .

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    Embryonic Stem Cells00:58

    Embryonic Stem Cells

    Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
    Embryonic Stem Cells00:57

    Embryonic Stem Cells

    Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
    ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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