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Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
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Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila

Richard W Carthew

    CSH Protocols
    |April 10, 2012
    PubMed
    Abstract

    No abstract available in PubMed .

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    In-situ Hybridization02:31

    In-situ Hybridization

    In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
    Types of probes and labels
    A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...