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Fixing Caenorhabditis elegans in Bouin's fixative

Ed Harlow, David Lane

    CSH Protocols
    |April 10, 2012
    PubMed

    Abstract:

    INTRODUCTIONBouin's fixative is a particularly good choice for worms because it penetrates dense tissues well and is extremely good for fixing antigens. Like all strong fixatives, however, it is unsuitable for some antibody-antigen pairs. In such cases, the length of time in the Bouin's fixative can be shortened, or paraformaldehyde fixation can be used instead.

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