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Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection
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Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection

Published on: January 19, 2024

Antibody addition to Drosophila specimens and detection using fluorochrome-linked reagents

Ed Harlow, David Lane

    CSH Protocols
    |April 10, 2012
    PubMed

    Abstract:

    INTRODUCTIONFluorochrome-linked reagents should be used when high resolution is needed or if two antigens need to be localized simultaneously. Because of the thickness of fly specimens, detection requires access to a confocal microscope.

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