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Related Concept Videos

SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

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Related Experiment Video

Updated: May 23, 2026

Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
08:44

Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)

Published on: October 29, 2009

Drying SDS-Polyacrylamide Gels.

Joseph Sambrook, David W Russell

    CSH Protocols
    |April 10, 2012
    PubMed
    Summary
    This summary is machine-generated.

    This protocol details a method for drying SDS-polyacrylamide gels, essential for obtaining autoradiographic images of radiolabeled proteins and preserving nonradioactive gels.

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    Published on: February 25, 2021

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Biotechnology

    Background:

    • SDS-polyacrylamide gel electrophoresis (SDS-PAGE) is a common technique for protein separation.
    • Autoradiography requires dried gels for clear imaging of radiolabeled proteins.
    • Preservation of protein gels is often necessary for long-term storage and analysis.

    Purpose of the Study:

    • To describe a standardized protocol for drying SDS-polyacrylamide gels.
    • To enable the acquisition of autoradiographic images from (35)S-labeled protein gels.
    • To provide a method for preserving both radioactive and nonradioactive protein gels.

    Main Methods:

    • The protocol involves specific steps for gel preparation prior to drying.
    • Utilizes a gel dryer apparatus to remove moisture from the polyacrylamide gel.
    • Details drying times and temperature parameters for optimal results.

    Main Results:

    • Successfully dried SDS-polyacrylamide gels suitable for downstream analysis.
    • Achieved clear autoradiographic images from dried gels containing (35)S-labeled proteins.
    • Demonstrated the preservation of gel integrity after the drying process.

    Conclusions:

    • The described method provides an effective means to dry SDS-polyacrylamide gels.
    • This protocol is crucial for researchers utilizing (35)S radiolabeling and autoradiography.
    • The drying technique ensures the long-term stability and usability of protein gels.