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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

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Related Experiment Video

Updated: May 23, 2026

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

Fluorescent Staining of Proteins with SYPRO Ruby.

Richard J Simpson

    CSH Protocols
    |April 10, 2012
    PubMed
    Summary

    SYPRO Ruby is a sensitive ruthenium-based fluorescent dye compatible with MALDI mass spectrometry. Its high cost may limit its use to smaller protein analysis applications.

    Area of Science:

    • Biochemistry
    • Proteomics
    • Analytical Chemistry

    Background:

    • SYPRO Ruby is a ruthenium-based fluorescent dye used for protein detection in gels.
    • It interacts with basic amino acids, offering sensitivity comparable to silver staining.
    • The dye is compatible with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry.

    Purpose of the Study:

    • To evaluate SYPRO Ruby as a sensitive protein staining method.
    • To assess its compatibility with MALDI mass spectrometry.
    • To discuss its potential limitations in large-scale quantitative studies.

    Main Methods:

    • Protein gel electrophoresis.
    • Staining with SYPRO Ruby (fixation, staining, destaining).
    • Analysis using MALDI mass spectrometry.

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    Staining Proteins in Gels
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    Published on: April 25, 2021

    Related Experiment Videos

    Last Updated: May 23, 2026

    Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
    06:24

    Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

    Published on: April 21, 2019

    Staining Proteins in Gels
    10:55

    Staining Proteins in Gels

    Published on: July 8, 2008

    Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
    14:04

    Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

    Published on: April 25, 2021

    Main Results:

    • SYPRO Ruby demonstrates high sensitivity, similar to silver staining.
    • The staining protocol is simple and efficient.
    • Complete compatibility with MALDI mass spectrometry was confirmed.

    Conclusions:

    • SYPRO Ruby is a sensitive and MALDI-compatible fluorescent stain for proteins.
    • Its three-step protocol is straightforward.
    • The high cost of SYPRO Ruby may restrict its application in large-scale quantitative proteomics.