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Radioisotopic Labeling of Eukaryotic Cell Proteins Using 32P

Hong Ji

    CSH Protocols
    |April 10, 2012
    PubMed

    Abstract:

    INTRODUCTIONRadioisotope labeling of proteins is an important technique in expression proteomics studies for detecting low-abundance proteins. The procedure given below describes a labeling technique using (32)P labeling. After the labeling procedure described here, cell lysates can be prepared for analysis by 2D gel electrophoresis.

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