Selection of appropriate imaging equipment and methodology for live cell imaging in Drosophila

The wide range of microscopy equipment and techniques are truly bewildering and ever-expanding. In the past two decades, there have been many revolutions in light microscopy techniques made possible by improvements in optics, detector technology, and computers. Furthermore, there is no indication that the rate of development of new equipment is slowing down. Here we attempt to provide an overview of available options and important considerations applicable to imaging Drosophila cells and tissues.