Related Experiment Video
Updated: May 23, 2026

09:26
FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.
Published on: August 25, 2020
Imaging protein interactions by FRET microscopy: FLIM measurements
CSH Protocols
|April 10, 2012
Abstract:
This image acquisition protocol is a basic plan for taking a fluorescence lifetime imaging (FLIM) series. FLIM makes live-cell FRET measurements based only on donor fluorescence more feasible, because lifetimes are independent of probe concentration and light path length. The former is not easy to determine in cells, and the latter means that cell shape is not a factor.

